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Spectrum Chromatography Toll-Free: 800/459-9700 Phone: +1-281-443-2900 Fax: +1-281-443-3100 Email: sales@spectra-chrom.com |
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The pump is generally one of the first things encountered by
the solvent in a chromatographic separation. Peristaltic
pumps are the most commonly used pump type for low pressure
liquid chromatography. In these pumps, the only wetted materials
are a piece of tubing, generally the same tubing used elsewhere
in the system; this limits the number of materials that the
solvent contacts.
Peristaltic pumps operate by pinching closed a point on a piece of tubing. This pinched point is then moved along the tubing to force the liquid to move. Using this model, the pump rate obviously depends upon both the size of the tubing and the speed that the pinch point moves.
As a general convenience to provide for maximum flexibility in choosing a pumping system, most peristaltic pump heads (the part that holds the tubing and pinches it shut) are sold separately from the pump drive (the part that has the motor and makes the pump head work.) This separation allows you to independently control the tubing size and the drive speed allowing you to select a tubing size convenient for you and a flow rate suitable for your chromatography.
We manufacture 3 different pump drives and a variety of pump heads for use in low-pressure liquid chromatography. These same items are also frequently use in general-purpose laboratory pumps.
The 3 pump drives we manufacture are the MP-1 (part number 124868), MP-2 (part number 146821), and Macroflow (part number 146800) drives. The MP-1 and MP-2 drives can accept up to 3 pump heads while the Macroflow drive can accept a maximum of 2 pump heads. The MP-1 and MP-2 pump drives also have remote control capabilities which allow them to be stopped remotely (e.g. by a CF-1 fraction collector at the end of a run).
Two different styles of pump heads are available. The most common pump head is the Fast-Load pump head. This pump head accepts any flexible tubing with a wall thickness of 1/16" (1.6 mm) and an outer diameter of 7/16" (11.1 mm) or less. The tubing can be loaded into this pump head without removing the pump head from the pump.
The second style of pump head is the standard pump head. The standard pump heads each accept only a single size of tubing. To load or unload the tubing from the pump head, the pump head must first be removed from the pump.
| MP-1 | MP-2 | Macroflow | |
|---|---|---|---|
| Pump Speed | to 60 rpm | to 600 rpm | to 600 rpm |
| Power Requirements | 100 VA |
400 VA |
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| Line Frequency | 45 to 65 Hz | ||
| Dimensions (without pump heads) w x h x l |
5.4" x 9.3" x 12.3" 14 x 24 x 31 cm |
5.4" x 9.3" x 12.3" 14 x 24 x 31 cm |
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| Operating Temperature | 0°C to 40°C, noncondensing | ||
| Tubing Number | 13 | 14 | 16 | 17 | 18 | 15 |
|---|---|---|---|---|---|---|
| Tubing Size (OD x ID) |
5/32" x
1/32" (4 x 0.8 mm) |
3/16" x
1/16" (4.8 x 1.6 mm) |
1/4" x
1/8" (6.3 x 3 mm) |
3/8" x
1/4" (9.5 x 6.3 mm) |
7/16" x
5/16" (11 x 8 mm) |
3/8" x
3/16" (9.5 x 4.8 mm) |
| Wall Thickness | 1/16" (1.6 mm) |
1/16" (1.6 mm) |
1/16" (1.6 mm) |
1/16" (1.6 mm) |
1/16" (1.6 mm) |
3/32" (2.4 mm) |
| Flow Rate with MP-1 Pump (ml/min) | 0.03 - 3.6 | 0.11 - 12.6 | 0.4 - 48 | 1.4 - 168 | 1.9 - 228 | 0.83 - 100 |
| Flow Rate with MP-2 Pump (ml/min) | 0.36 - 36 | 1.3 - 126 | 5 - 480 | 17 - 1680 | 23 - 2280 | 10 - 1000 |
| Flow Rate with Macroflow Pump (ml/min) | 0.72 - 36 | 2.5 - 126 | 20 - 480 | 35 - 1680 | 45 - 2280 | 20 - 1000 |
| Recommended Pump Head | Fast Load 146924 |
Fast Load 146924 |
Fast Load 146924 |
Fast Load 146924 |
Fast Load 146924 |
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| Optional Pump Head | Standard 146809 |
Standard 146811 |
Standard 146813 |
Standard 146817 |
Standard 146819 |
Standard 146815 |
| Tubing Part Numbers: | ||||||
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Silicone, 3m Silicone, 15m Vinyl, 3m Vinyl, 30m LPF, 3m |
123732 123734 123728 123730 123736 |
123746 123748 123742 123744 123750 |
123760 123762 123756 123758 123764 |
123856 123858 123852 123854 123860 |
123788 123790 123784 123786 123792 |
123842 123844 123838 123840 123846 |
Additional mounting hardware is required to mount 2 or 3 pump heads to a pump drive. Part number 146823 is required to mount 2 pump heads and part number 146828 is required to mount 3 pump heads.
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